Show Changes Show Changes
Edit Edit
Print Print
Recent Changes Recent Changes
Subscriptions Subscriptions
Lost and Found Lost and Found
Find References Find References
Rename Rename
Search

History

1/8/2009 11:26:16 AM
BC-spneajt
1/8/2009 11:25:40 AM
BC-spneajt
1/8/2009 11:25:19 AM
BC-spneajt
1/8/2009 11:24:37 AM
BC-spneajt
1/8/2009 10:49:04 AM
BC-spneajt
List all versions List all versions
Protein Phosphorylation Tools
.

Summary

This page describes some tools freely available on the web to help identify potential and actual phosphorylation sites on proteins.

Phosphositeplus

Websitehttp://www.phosphosite.org/homeAction.do
DescriptionA manually curated compendium of known protein phosphorylation sites provided by Cell Signalling Technology. Users can enter a protein name to search for known phosphorylation sites or alternatively search via a kinase of interest for known substrates.

Phospho.ELM

Websitehttp://phospho.elm.eu.org/
DescriptionThis is a manually curated compendium of known protein phosphorylation sites. Users can enter a protein name in Uniprot format from http://www.uniprot.org/ and search for known phosphorylation sites with the option of filtering through an extensive list of kinases and/or binding motifs. The results are reported together with Swiss-Prot protein identification and Pub-Med reference links. Additional information can include the kinase, when known, and homology domains determined from SMART/Pfam. The current release (Version 5.0 May 2006) contains 2540 protein entries covering 7206 instances. Note that this search will not predict new potential phosphorylation sites.
References"Phospho.ELM: a database of experimentally verified phosphorylation sites in eukaryotic proteins." Diella F, Cameron S, Gemund C, Linding R, Via A, Kuster B, Sicheritz-Ponten T, Blom N, Gibson TJ. BMC Bioinformatics. 2004 Jun 22;5:79.

Netphos 2.0 Server

Websitehttp://www.cbs.dtu.dk/services/NetPhos/
DescriptionThis is a neural network-based tool that scores potential phosphorylation sites in a probabilistic manner.
References"Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites." Blom, N., Gammeltoft, S., and Brunak, S. Journal of Molecular Biology: 294: 1351-1362, 1999.

Netphosk 1.0 Server

Websitehttp://www.cbs.dtu.dk/services/NetPhosk/
DescriptionThis tool searches protein sequences for potential (predicted) kinase-specific phosphorylation sites based on neural network-derived kinase recognition consensus sequences. Users paste the protein sequence of interest in FASTA format, which can be obtained from the Uniprot database (http://www.uniprot.org/). The kinases currently enabled for the search are: ATM CaM-II Cdc2 Cdk5 CKI CKII DNA EGFR Gsk3 INSR MAPK p38 PK PKA PKB PKC PKG RSK Src
References"Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence." Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S, Brunak S. Proteomics. 2004 Jun; 4:1633-49. Review.

How to create and edit pages

Recent Topics

Protein Phosphorylation Tools